The differential regulation of osteoblast and osteoclast activity by surface topography of hydroxyapatite coatings

被引:230
作者
Costa, Daniel O. [1 ]
Prowse, Paul D. H. [2 ]
Chrones, Tom [3 ]
Sims, Stephen M. [3 ]
Hamilton, Douglas W. [2 ,4 ]
Rizkalla, Amin S. [1 ,2 ,4 ]
Dixon, S. Jeffrey [3 ]
机构
[1] Univ Western Ontario, Fac Engn, Dept Chem & Biochem Engn, London, ON N6A 5B9, Canada
[2] Univ Western Ontario, Biomed Engn Program, London, ON N6A 5B9, Canada
[3] Univ Western Ontario, Schulich Sch Med & Dent, Dept Physiol & Pharmacol, London, ON N6A 5C1, Canada
[4] Univ Western Ontario, Schulich Sch Med & Dent, Schulich Dent, London, ON N6A 5C1, Canada
基金
加拿大自然科学与工程研究理事会; 加拿大健康研究院;
关键词
Biomimetic material; Hydroxyapatite coating; Osteoblast; Osteoclast; Surface roughness; Surface topography; BONE-LIKE APATITE; CALCIUM-PHOSPHATE; RESORPTION; ADHESION; CELLS; ROUGHNESS; MICROTOPOGRAPHY; BIOMATERIALS; PROLIFERATION; ORGANIZATION;
D O I
10.1016/j.biomaterials.2013.06.014
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
The behavior of bone cells is influenced by the surface chemistry and topography of implants and scaffolds. Our purpose was to investigate how the topography of biomimetic hydroxyapatite (HA) coatings influences the attachment and differentiation of osteoblasts, and the resorptive activity of osteoclasts. Using strategies reported previously, we directly controlled the surface topography of HA coatings on polycaprolactone discs. Osteoblasts and osteoclasts were incubated on HA coatings having distinct isotropic topographies with submicrometer and micro-scale features. Osteoblast attachment and differentiation were greater on more complex, micro-rough HA surfaces (Ra similar to 2 mu m) than on smoother topographies (Ra similar to 1 mu m). In contrast, activity of the osteoclast marker tartrate-resistant acid phosphatase was greater on smoother than on micro-rough surfaces. Furthermore, scanning electron microscopy revealed the presence of resorption lacunae exclusively on smoother HA coatings. Inhibition of resorption on micro-rough surfaces was associated with disruption of filamentous actin sealing zones. In conclusion, HA coatings can be prepared with distinct topographies, which differentially regulate responses of osteoblasts, as well as osteoclastic activity and hence susceptibility to resorption. Thus, it may be possible to design HA coatings that induce optimal rates of bone formation and degradation specifically tailored for different applications in orthopedics and dentistry. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:7215 / 7226
页数:12
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