Global gene expression and functional network analysis of gastric cancer identify extended pathway maps and GPRC5A as a potential biomarker

被引：49

作者：

Cheng, Lei ^{[1
,2
,3
]}

Yang, Sheng ^{[2
,3
,5
]}

Yang, Yanqing ^{[2
,3
]}

Zhang, Wen ^{[1
]}

Xiao, Huasheng ^{[1
]}

Gao, Hengjun ^{[1
,6
]}

Deng, Xiaxing ^{[4
]}

Zhang, Qinghua ^{[2
,3
,7
]}

机构：

[1] Natl Engn Ctr Biochip Shanghai, Shanghai, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, State Key Lab Med Genom, Shanghai, Peoples R China

[3] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Shanghai Inst Hematol, Shanghai, Peoples R China

[4] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Gen Surg, Shanghai, Peoples R China

[5] Lab Shanghai Skin Dis Hosp, Shanghai, Peoples R China

[6] Tongji Univ, Tongji Hosp, Inst Digest Dis, Shanghai 200092, Peoples R China

[7] Wayen Biotechnol Inc, Shanghai, Peoples R China

来源：

CANCER LETTERS | 2012年 / 326卷 / 01期

关键词：

Gastric cancer; Expression microarray; Network analysis; Pathway; Biomarker; STEM-CELL ANTIGEN; SHOCK-PROTEIN; 47; COPY NUMBER; IDENTIFICATION; TARGET; CARCINOMA; SURVIVAL; BREAST; PSCA; OVEREXPRESSION;

D O I：

10.1016/j.canlet.2012.07.031

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To get more understanding of the molecular mechanisms underlying gastric cancer, 25 paired samples were applied to gene expression microarray analysis. Here, expression microarray, quantitative reverse transcription-PCR (qRT-PCR) and immunohistochemical analysis indicated that GPRC5A was significantly elevated in gastric cancer tissues. The integrative network analysis of deregulated genes generated eight subnetworks. We also mapped copy number variations (CNVs) and associated mRNA expression changes into pathways and identified WNT, RTK-Ras-PI3K-AKT, NF-kappa B, and PLAU-JAK-STAT pathways involved in proliferation, evading apoptosis and sustained angiogenesis, respectively. Taken together, our results reveal several interesting genes including GPRC5A as potential biomarkers for gastric cancer, and highlight more systematical insight of deregulated genes in genetic pathways of gastric carcinogenesis. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：105 / 113

页数：9

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