A Ratiometric Fluorescent Probe Based on FRET for Imaging Hg2+ Ions in Living Cells

被引：797

作者：

Zhang, Xiaolin ^{[1
]}

Xiao, Yi ^{[1
]}

Qian, Xuhong ^{[2
]}

机构：

[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China

[2] E China Univ Sci & Technol, Biol Chem Lab, Shanghai 200237, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 42期

关键词：

fluorescent probes; FRET; mercury; rhodamine; sensors;

D O I：

10.1002/anie.200803246

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Mercury rising: A FRET-based ratiometric probe (see picture; FRET = fluorescence resonance energy transfer) can selectively detect Hg2+ ions on the parts-per-billion scale under physiological conditions. The probe undergoes a clear Hg2+-induced change in the intensity ratio ofthe two well-separated and equally intense emission bands of dipyrrometheneboron difluoride and rhodamine. Experiments show that the probe can be used in living cells. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：8025 / 8029

页数：5

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