Reverse transcriptase-mediated tropism switching in Bordetella bacteriophage

被引：192

作者：

Liu, MS

Deora, R

Doulatov, SR

Gingery, M

Eiserling, FA

Preston, A

Maskell, DJ

Simons, RW

Cotter, PA

Parkhill, J

Miller, JF ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Microbiol Mol Genet & Immunol, Los Angeles, CA 90095 USA

[2] Univ Cambridge, Dept Clin Vet Med, Ctr Vet Sci, Cambridge CB3 0ES, England

[3] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA

[4] Sanger Ctr, Cambridge, England

来源：

SCIENCE | 2002年 / 295卷 / 5562期

关键词：

D O I：

10.1126/science.1067467

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Host-pathogen interactions are often driven by mechanisms that promote genetic variability. We have identified a group of temperate bacteriophages that generate diversity in a gene, designated mtd (major tropism determinant), which specifies tropism for receptor molecules on host Bordetella species. Tropism switching is the result of a template-dependent, reverse transcriptase-mediated process that introduces nucleotide substitutions at defined locations within mtd. This cassette-based mechanism is capable of providing a vast repertoire of potential ligand-receptor interactions.

引用

页码：2091 / 2094

页数：4

共 15 条

[1] ECTOPIC EXPRESSION OF THE FLAGELLAR REGULON ALTERS DEVELOPMENT OF THE BORDETELLA HOST INTERACTION [J].

AKERLEY, BJ ;

COTTER, PA ;

MILLER, JF .

CELL, 1995, 80 (04) :611-620

[2] A mutation in the Bordetella bronchiseptica bvgS gene results in reduced virulence and increased resistance to starvation, and identifies a new class of Bvg-regulated antigens [J].

Cotter, PA ;

Miller, JF .

MOLECULAR MICROBIOLOGY, 1997, 24 (04) :671-685

[3]

COTTER PA, 2001, PRINCIPLES BACTERIAL, P620

[4] Diversity in the Bordetella virulence regulon:: transcriptional control of a Bvg-intermediate phase gene [J].

Deora, R ;

Bootsma, HJ ;

Miller, JF ;

Cotter, PA .

MOLECULAR MICROBIOLOGY, 2001, 40 (03) :669-683

[5]

DOI H, 1991, P NATL ACAD SCI USA, V88, P9282, DOI 10.1073/pnas.88.20.9282

[6]

DOULATOV S, UNPUB

[7] Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression [J].

Edwards, RA ;

Keller, LH ;

Schifferli, DM .

GENE, 1998, 207 (02) :149-157

[8] RNA editing by base deamination: more enzymes, more targets, more mysteries [J].

Gerber, AP ;

Keller, W .

TRENDS IN BIOCHEMICAL SCIENCES, 2001, 26 (06) :376-384

[9] Initial sequencing and analysis of the human genome [J].

Lander, ES ;

Int Human Genome Sequencing Consortium ;

Linton, LM ;

Birren, B ;

Nusbaum, C ;

Zody, MC ;

Baldwin, J ;

Devon, K ;

Dewar, K ;

Doyle, M ;

FitzHugh, W ;

Funke, R ;

Gage, D ;

Harris, K ;

Heaford, A ;

Howland, J ;

Kann, L ;

Lehoczky, J ;

LeVine, R ;

McEwan, P ;

McKernan, K ;

Meldrim, J ;

Mesirov, JP ;

Miranda, C ;

Morris, W ;

Naylor, J ;

Raymond, C ;

Rosetti, M ;

Santos, R ;

Sheridan, A ;

Sougnez, C ;

Stange-Thomann, N ;

Stojanovic, N ;

Subramanian, A ;

Wyman, D ;

Rogers, J ;

Sulston, J ;

Ainscough, R ;

Beck, S ;

Bentley, D ;

Burton, J ;

Clee, C ;

Carter, N ;

Coulson, A ;

Deadman, R ;

Deloukas, P ;

Dunham, A ;

Dunham, I ;

Durbin, R ;

French, L .

NATURE, 2001, 409 (6822) :860-921

[10] PERTACTIN, AN ARG-GLY-ASP-CONTAINING BORDETELLA-PERTUSSIS SURFACE PROTEIN THAT PROMOTES ADHERENCE OF MAMMALIAN-CELLS [J].

LEININGER, E ;

ROBERTS, M ;

KENIMER, JG ;

CHARLES, IG ;

FAIRWEATHER, N ;

NOVOTNY, P ;

BRENNAN, MJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (02) :345-349

← 1 2 →