A crucial role in cell spreading for the interaction of Abl PxxP motifs with Crk and Nck adaptors

被引：41

作者：

Antoku, Susumu ^{[1
]}

Saksela, Kalle ^{[2
,3
]}

Rivera, Gonzalo M. ^{[1
]}

Mayer, Bruce J. ^{[1
]}

机构：

[1] Univ Connecticut, Ctr Hlth, Dept Genet & Dev Biol, Raymond & Beverly Sackler Lab Genet & Mol Med, Farmington, CT 06030 USA

[2] Univ Helsinki, Haartman Inst, Dept Virol, FIN-00014 Helsinki, Finland

[3] Univ Helsinki, Cent Hosp, FIN-00014 Helsinki, Finland

来源：

JOURNAL OF CELL SCIENCE | 2008年 / 121卷 / 18期

基金：

美国国家卫生研究院; 芬兰科学院;

关键词：

Abl PxxP motifs; Crk; Nck; filopodium and lamellipodium; cell spreading;

D O I：

10.1242/jcs.031575

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The dynamic reorganization of actin structures helps to mediate the interaction of cells with their environment. The Abl nonreceptor tyrosine kinase can modulate actin rearrangement during cell attachment. Here we report that the Abl PxxP motifs, which bind Src homology 3 (SH3) domains, are indispensable for the coordinated regulation of filopodium and focal adhesion formation and cell-spreading dynamics during attachment. Candidate Abl PxxP-motif-binding partners were identified by screening a comprehensive SH3-domain phage-display library. A combination of protein overexpression, silencing, pharmacological manipulation and mutational analysis demonstrated that the PxxP motifs of Abl exert their effects on actin organization by two distinct mechanisms, involving the inhibition of Crk signaling and the engagement of Nck. These results uncover a previously unappreciated role for Abl PxxP motifs in the regulation of cell spreading.

引用

页码：3071 / 3082

页数：12

共 66 条

[11] IDENTIFICATION AND CHARACTERIZATION OF A HIGH-AFFINITY INTERACTION BETWEEN V-CRK AND TYROSINE-PHOSPHORYLATED PAXILLIN IN CT10-TRANSFORMED FIBROBLASTS [J].