Liquid chromatography-electrospray mass spectrometric identification of ginsenosides in Panax ginseng roots

被引：165

作者：

Fuzzati, N ^{[1
]}

Gabetta, B ^{[1
]}

Jayakar, K ^{[1
]}

Pace, R ^{[1
]}

Peterlongo, F ^{[1
]}

机构：

[1] Indena SPA, Lab Ric & Sviluppo, I-20090 Milan, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 854卷 / 1-2期

关键词：

Panax ginseng; plant materials; ginsenosides;

D O I：

10.1016/S0021-9673(99)00463-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method was developed for electrospray mass spectrometric analysis of ginsenosides in Panax ginseng roots. The analyses were performed on a reversed-phase C-18 column using a binary eluent (aqueous 8 mM NH4OAc, buffered to pH 7 with NH4OH-acetonitrile) under gradient conditions. Twenty-five ginsenosides could be separated and detected. The mass spectra obtained provided information on their molecular masses. A MS-MS experiment was undertaken in order to determine the sugar unit sequences and the aglycone moieties. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：69 / 79

页数：11

共 16 条

[1] GINSENOSIDE CONTENTS OF PLANTLETS REGENERATED FROM PANAX-GINSENG EMBRYOIDS
ASAKA, I
II, I
HIROTANI, M
ASADA, Y
FURUYA, T
[J]. PHYTOCHEMISTRY, 1994, 36 (01) : 61 - 63
[2] A COMPARATIVE-STUDY ON COMMERCIAL SAMPLES OF GINSENG-RADIX
CHUANG, WC
WU, HK
SHEU, SJ
CHIOU, SH
CHANG, HC
CHEN, YP
[J]. PLANTA MEDICA, 1995, 61 (05) : 459 - 465
[3] DETERMINATION OF GINSENOSIDES IN GINSENG CRUDE EXTRACTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
CHUANG, WC
SHEU, SJ
[J]. JOURNAL OF CHROMATOGRAPHY A, 1994, 685 (02) : 243 - 251
[4] Cole R.B., 1997, Electrospray Ionization Mass Spectrometry
[5] HATTORI M, 1988, CHEM PHARM BULL, V36, P4467
[6] HATTORI M, 1988, Shoyakugaku Zasshi, V42, P228
[7] Hostettmann K., 1995, SAPONINS CHEM PHARM, V59, P96
[8] COMPARISON OF COLUMNS OF CHEMICALLY MODIFIED POROUS-GLASS AND SILICA IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF GINSENOSIDES
KANAZAWA, H
NAGATA, Y
KUROSAKI, E
MATSUSHIMA, Y
TAKAI, N
[J]. JOURNAL OF CHROMATOGRAPHY, 1993, 632 (1-2): : 79 - 85
[9] KITAGAWA I, 1989, CHEM PHARM BULL, V37, P2961, DOI 10.1248/cpb.37.2961
[10] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF GINSENOSIDES USING PHOTOREDUCTION FLUORESCENCE DETECTION
PARK, MK
KIM, BK
PARK, JH
SHIN, YG
CHO, KH
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1995, 18 (10): : 2077 - 2088

← 1 2 →