Characterization of allergenic food proteins for improved diagnostic methods

被引:18
作者
Beyer, Kirsten [1 ]
机构
[1] Mt Sinai Sch Med, Div Pediat Allergy & Immunol, Box 1198,One Gustave Levy Pl, New York, NY 10029 USA
关键词
food allergy; allergens; diagnostic; IgE; Proteomics; microarray;
D O I
10.1097/01.all.0000072716.82112.85
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Purpose of review Diagnostic decision points for food allergen-specific serum IgE concentration and for skin prick test results have been established for several foods, reducing the requirement for food challenges in a number of patients. Many patients, however, still need to undergo oral food challenges because their food-specific IgE level is in the undefined range. In addition, diagnostic decision points could not be established for several foods. It appears that measurement of serum specific IgE concentrations to individual allergens is superior to determination of specific IgE to the crude food extract containing allergenic and nonallergenic proteins. This review will outline recent advantages in characterization of food allergens as well as the relevance of this knowledge for use in recently developed protein microarray technology. Recent findings Protein microarrays have been developed to profile allergen-specific IgE antibodies from human serum with the advantage of screening hundreds of allergens in parallel using minute amounts of blood. This technology, however, requires prior knowledge of the proteins to be studied. The identification and characterization of clinically relevant allergens have increased dramatically within the last several years. Relevant new allergens have been identified, especially in tree nuts and seeds. Interestingly, most of these allergens belong to the same family of seed storage proteins. In addition, known food allergens have been further characterized and IgE-binding sites have been determined. Moreover, 'informative' peptides shown to be predictive for the persistence of food allergy have been identified. Summary The combination of food allergen characterization and protein or peptide microarray technology will enable us to develop improved diagnostic tools in food allergy.
引用
收藏
页码:189 / 197
页数:9
相关论文
共 83 条
[61]   Improving in-vitro tests for the diagnosis of food hypersensitivity [J].
Sampson, Hugh A. .
CURRENT OPINION IN ALLERGY AND CLINICAL IMMUNOLOGY, 2002, 2 (03) :257-261
[62]  
Schocker F, 2002, J ALLERGY CLIN IMMUN, pS109
[63]   Measuring proteins on microarrays [J].
Schweitzer, B ;
Kingsmore, SF .
CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (01) :14-19
[64]   Protein structure plays a critical role in peanut allergen stability and may determine immunodominant IgE-binding epitopes [J].
Sen, M ;
Kopper, R ;
Pons, L ;
Abraham, EC ;
Burks, AW ;
Bannon, GA .
JOURNAL OF IMMUNOLOGY, 2002, 169 (02) :882-887
[65]   Biochemical and structural analysis of the IgE binding sites on Ara h1, an abundant and highly allergenic peanut protein [J].
Shin, DS ;
Compadre, CM ;
Maleki, SJ ;
Kopper, RA ;
Sampson, H ;
Huang, SK ;
Burks, AW ;
Bannon, GA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (22) :13753-13759
[66]   Use of a peptide microarray immunoassay for the analysis of IgE-binding epitopes of major peanut allergens [J].
Shreffler, WG ;
Beyer, K ;
Chu, T ;
Ellman, L ;
Stanley, S ;
Bannon, GA ;
Burks, W ;
Sampson, HA .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2002, 109 (01) :S286-S286
[67]   IgE binding to soluble and insoluble wheat flour proteins in atopic and non-atopic patients suffering from gastrointestinal symptoms after wheat ingestion [J].
Simonato, B ;
De Lazzari, F ;
Pasini, G ;
Polato, F ;
Giannattasio, M ;
Gemignani, C ;
Peruffo, ADB ;
Santucci, B ;
Plebani, M ;
Curioni, A .
CLINICAL AND EXPERIMENTAL ALLERGY, 2001, 31 (11) :1771-1778
[68]  
Sporik R, 2000, CLIN EXP ALLERGY, V30, P1540, DOI 10.1046/j.1365-2222.2000.00928.x
[69]   Allergy to peanut, nuts, and sesame seed in Australian children [J].
Sporik, R ;
Hill, D .
BRITISH MEDICAL JOURNAL, 1996, 313 (7070) :1477-1478
[70]   Identification and mutational analysis of the immunodominant IgE binding epitopes of the major peanut allergen Ara h 2 [J].
Stanley, JS ;
King, N ;
Burks, AW ;
Huang, SK ;
Sampson, H ;
Cockrell, G ;
Helm, RM ;
West, CM ;
Bannon, GA .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1997, 342 (02) :244-253