Activity and utility of abscisic acid having a 3' thioether linker arm

被引：4

作者：

Balsevich, JJ ^{[1
]}

Bishop, GG ^{[1
]}

Banowetz, GM ^{[1
]}

机构：

[1] USDA ARS, CORVALLIS, OR 97331 USA

来源：

PHYTOCHEMISTRY | 1997年 / 44卷 / 02期

关键词：

Lepidium sativum; abscisic acid; linker arm; affinity chromatography; antibody; analogues; preparation; cress; germination;

D O I：

10.1016/S0031-9422(96)00440-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abscisic acid (ABA) was functionalized at the 3'-position by reaction of its alpha-2',3'-epoxide with 1,4-butanedithiol to afford the corresponding derivative having a six-atom linker arm containing a terminal sulphhydryl group. This derivative was coupled with epoxy activated Sepharose 6B affording a gel containing ca 1.4 mg of (+)-ABA ml(-1). This gel was shown to bind specifically an anti-ABA antibody from hybridoma culture broth, and its binding capacity was determined. The (+)-ABA analogue possessing the thiol linker arm was also derivatized by reaction with a fluorescent derivatizing agent and these compounds were shown to inhibit cress seed germination. Copyright (C) 1996 Elsevier Science Ltd

引用

页码：215 / 220

页数：6

共 18 条

[1]

ALLAN AC, 1994, PLANT PHYSIOL, V104, P1107

[2] A MONOCLONAL-ANTIBODY AGAINST THE PLANT-GROWTH REGULATOR, ABSCISIC-ACID [J].