High Affinity scFv-Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells

被引：28

作者：

Iyer, Gopal ^{[1
]}

Michalet, Xavier ^{[1
]}

Chang, Yun-Pei ^{[1
]}

Pinaud, Fabien F. ^{[1
]}

Matyas, Stephanie E. ^{[2
]}

Payne, Gregory ^{[3
]}

Weiss, Shimon ^{[1
,4
]}

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90024 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Hematol Oncol, Los Angeles, CA 90024 USA

[3] Univ Calif Los Angeles, David Geffen Sch Med, Dept Biol Chem, Los Angeles, CA 90024 USA

[4] Univ Calif Los Angeles, David Geffen Sch Med, Dept Physiol, Los Angeles, CA 90024 USA

来源：

NANO LETTERS | 2008年 / 8卷 / 12期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1021/nl8032284

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe a general approach to label cell surface proteins using quantum dots (CID) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a.

引用

页码：4618 / 4623

页数：6

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