16S rRNA gene and lipid biomarker evidence for anaerobic ammonium-oxidizing bacteria (anammox) in California and Nevada hot springs

被引：83

作者：

Jaeschke, Andrea ^{[1
]}

den Camp, Huub J. M. Op ^{[2
]}

Harhangi, Harry ^{[2
]}

Klimiuk, Adam ^{[1
]}

Hopmans, Ellen C. ^{[1
]}

Jetten, Mike S. M. ^{[2
]}

Schouten, Stefan ^{[1
]}

Damste, Jaap S. Sinninghe ^{[1
]}

机构：

[1] NIOZ Royal Netherlands Inst Sea Res, Dept Marine Organ Biogeochem, NL-1790 AB Den Burg, Texel, Netherlands

[2] Radboud Univ Nijmegen, Inst Water & Wetland Res, Dept Microbiol, Nijmegen, Netherlands

来源：

FEMS MICROBIOLOGY ECOLOGY | 2009年 / 67卷 / 03期

关键词：

anammox; hot springs; ladderane lipids; 16S rRNA gene; microorganisms; INTRACYTOPLASMIC COMPARTMENT; N-2; PRODUCTION; DIVERSITY; OXIDATION; ARCHAEAL; CRENARCHAEOTA; PHYLOGENIES; SEDIMENTS; SULFIDE; SYSTEM;

D O I：

10.1111/j.1574-6941.2008.00640.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Anammox, the oxidation of ammonium with nitrite to dinitrogen gas under anoxic conditions, is an important process in mesophilic environments such as wastewaters, oceans and freshwater systems, but little is known of this process at elevated temperatures. In this study, we investigated anammox in microbial mats and sediments obtained from several hot springs in California and Nevada, using geochemical and molecular microbiological methods. Anammox bacteria-specific ladderane core lipids with concentrations ranging between 0.3 and 52 ng g(-1) sediment were detected in five hot springs analyzed with temperatures up to 65 degrees C. In addition, 16S rRNA gene analysis showed the presence of genes phylogenetically related to the known anammox bacteria Candidatus Brocadia fulgida, Candidatus Brocadia anammoxidans and Candidatus Kuenenia stuttgartiensis (96.5-99.8% sequence identity) in three hot springs with temperatures up to 52 degrees C. Our data indicate that anammox bacteria may be able to thrive at thermophilic temperatures and thus may play a significant role in the nitrogen cycle of hot spring environments.

引用

页码：343 / 350

页数：8

共 46 条

[1] Marine microorganisms and global nutrient cycles [J].