The pH sensitivity of H2O2 metabolism in skeletal muscle mitochondria

被引：30

作者：

Banh, Sheena ^{[1
]}

Treberg, Jason R. ^{[1
,2
]}

机构：

[1] Univ Manitoba, Dept Biol Sci, Winnipeg, MB, Canada

[2] Univ Manitoba, Dept Human Nutr Sci, Winnipeg, MB, Canada

来源：

FEBS LETTERS | 2013年 / 587卷 / 12期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Reactive oxygen species; Mitochondria; Peroxidase; Antioxidant; Muscle; SUPEROXIDE-PRODUCTION; COMPLEX-I; BRAIN MITOCHONDRIA; OXIDATIVE STRESS; REMOVAL; PROTON; SITES; NADH;

D O I：

10.1016/j.febslet.2013.04.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Mitochondria have the capacity to produce and consume H2O2. We examined H2O2 metabolism in isolated rat skeletal muscle mitochondria and found that the substrate-dependent capacity to consume extramitochondrial H2O2 was markedly higher than the observed rate of H2O2 efflux from mitochondria under the same conditions. The substrate-dependent capacity to consume H2O2 was sensitive to the pH of the medium and we propose that pH related differences in H2O2 consumption pathways may explain inconsistencies we observed between H2O2 efflux rate and the reduction state of the matrix NADH pool. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1799 / 1804

页数：6

共 30 条

[1]

Affourtit C, 2012, METHODS MOL BIOL, V810, P165, DOI 10.1007/978-1-61779-382-0_11

[2]

Mitochondrial metabolism of reactive oxygen species [J].