Measurement of H2O2 within Living Drosophila during Aging Using a Ratiometric Mass Spectrometry Probe Targeted to the Mitochondrial Matrix

被引：252

作者：

Cocheme, Helena M. ^{[1
,2
]}

Quin, Caroline ^{[4
]}

McQuaker, Stephen J. ^{[4
]}

Cabreiro, Filipe ^{[2
]}

Logan, Angela ^{[1
]}

Prime, Tracy A. ^{[1
]}

Abakumova, Irina ^{[1
]}

Patel, Jigna V. ^{[2
]}

Fearnley, Ian M. ^{[1
]}

James, Andrew M. ^{[1
]}

Porteous, Carolyn M. ^{[5
,6
]}

Smith, Robin A. J. ^{[5
]}

Saeed, Saima ^{[3
]}

Carre, Jane E. ^{[3
]}

Singer, Mervyn ^{[3
]}

Gems, David ^{[2
]}

Hartley, Richard C. ^{[4
]}

Partridge, Linda ^{[2
]}

Murphy, Michael P. ^{[1
]}

机构：

[1] MRC, Mitochondrial Biol Unit, Cambridge CB2 0XY, England

[2] UCL, Inst Healthy Ageing, Dept Genet Evolut & Environm, London WC1E 6BT, England

[3] UCL, Dept Med, Bloomsbury Inst Intens Care Med, London WC1E 6BT, England

[4] Univ Glasgow, WestCHEM Sch Chem, Ctr Chem Res Ageing, Glasgow G12 8QQ, Lanark, Scotland

[5] Univ Otago, Dept Chem, Dunedin 9054, New Zealand

[6] Univ Otago, Dept Biochem, Dunedin 9054, New Zealand

来源：

CELL METABOLISM | 2011年 / 13卷 / 03期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金; 英国医学研究理事会;

关键词：

HYDROGEN-PEROXIDE; FREE-RADICALS; CATIONS; CELLS; RESTRICTION; INVOLVEMENT; ACTIVATION; DAMAGE;

D O I：

10.1016/j.cmet.2011.02.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Hydrogen peroxide (H2O2) is central to mitochondrial oxidative damage and redox signaling, but its roles are poorly understood due to the difficulty of measuring mitochondrial H2O2 in vivo. Here we report a ratiometric mass spectrometry probe approach to assess mitochondrial matrix H2O2 levels in vivo. The probe, MitoB, comprises a triphenylphosphonium (TPP) cation driving its accumulation within mitochondria, conjugated to an arylboronic acid that reacts with H2O2 to form a phenol, MitoP. Quantifying the MitoP/MitoB ratio by liquid chromatographytandem mass spectrometry enabled measurement of a weighted average of mitochondria! H2O2 that predominantly reports on thoracic muscle mitochondria within living flies. There was an increase in mitochondrial H2O2 with age in flies, which was not coordinately altered by interventions that modulated life span. Our findings provide approaches to investigate mitochondrial ROS in vivo and suggest that while an increase in overall mitochondrial H2O2 correlates with aging, it may not be causative.

引用

页码：340 / 350

页数：11

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