A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells

被引：566

作者：

Dickinson, Bryan C. ^{[1
]}

Chang, Christopher J. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 30期

关键词：

D O I：

10.1021/ja802355u

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present the design, synthesis, and biological applications of mitochondria peroxy yellow 1 (MitoPY1), a new type of bifunctional fluorescent probe for imaging hydrogen peroxide levels within the mitochondria of living cells. MitoPY1 combines a chemoselective boronate-based switch and a mitochondrial-targeting phosphonium moiety for detection of hydrogen peroxide localized to cellular mitochondria. Confocal microscopy and flow cytometry experiments in a variety of mammalian cell types show that MitoPY1 can visualize localized changes in mitochondrial hydrogen peroxide concentrations generated by situations of oxidative stress.

引用

页码：9638 / +

页数：3

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