Mito-DEPMPO synthesized from a novel NH2-reactive DEPMPO spin trap:: a new and improved trap for the detection of superoxide

被引：41

作者：

Hardy, Micael

Chalier, Florence

Ouari, Olivier

Finet, Jean-Pierre

Rockenbauer, Antal

Kalyanaraman, Balaraman

Tordo, Paul ^{[1
]}

机构：

[1] CNRS, SREP, UMR6517, F-13013 Marseille, France

[2] Univ Aix Marseille 1, Ctr St Jerome, F-13013 Marseille, France

[3] Univ Aix Marseille 2, Ctr St Jerome, F-13013 Marseille, France

[4] Univ Aix Marseille 3, Ctr St Jerome, F-13013 Marseille, France

[5] Inst Struct Chem, Chem Res Ctr, H-1525 Budapest, Hungary

[6] Med Coll Wisconsin, Dept Biophys, Milwaukee, WI 53226 USA

[7] Med Coll Wisconsin, Free Radical Res Ctr, Milwaukee, WI 53226 USA

来源：

CHEMICAL COMMUNICATIONS | 2007年 / 10期

关键词：

D O I：

10.1039/b616076j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Mito-DEPMPO, a new DEPMPO analogue bearing a triphenylphosphonium group, was synthesized via a novel NH2-reactive DEPMPO. The half-life of the Mito-DEPMPO superoxide adduct was estimated to be ca. 40 min. Using Mito-DEPMPO, reactive oxygen species generated in intact mitochondria were detected and characterized by EPR.

引用

页码：1083 / 1085

页数：3

共 32 条

[1] ESR study of spin-trapping with two glycosylated analogues of PBN able to target cell membrane lectins [J].