Virus-like particle vaccine induces cross-protection against human metapneumovirus infections in mice

被引：34

作者：

Levy, Camille ^{[1
,2
,3
,4
,5
,6
]}

Aerts, Laetitia ^{[7
,8
]}

Hamelin, Marie-Eve ^{[7
,8
]}

Granier, Christelle ^{[1
,2
,3
,4
,5
,6
]}

Szecsi, Judit ^{[1
,2
,3
,4
,5
]}

Lavillette, Dimitri ^{[1
,2
,3
,4
,5
]}

Boivin, Guy ^{[7
,8
]}

Cosset, Francois-Loic ^{[1
,2
,3
,4
,5
,6
]}

机构：

[1] Univ Lyon, EVIR Team, Int Ctr Infectiol Res, CIRI, F-69007 Lyon, France

[2] INSERM, U1111, F-69008 Lyon, France

[3] Ecole Normale Super Lyon, F-69364 Lyon, France

[4] Univ Lyon 1, Ctr Int Rech Infectiol, F-69365 Lyon, France

[5] CNRS, UMR5308, Lyon, France

[6] Univ Lyon, LabEx Ecofect, F-69007 Lyon, France

[7] Ctr Hosp Univ Quebec, Ctr Rech Infectiol, Quebec City, PQ G1V 4G2, Canada

[8] Univ Laval, Quebec City, PQ G1V 4G2, Canada

来源：

VACCINE | 2013年 / 31卷 / 25期

基金：

加拿大健康研究院; 欧洲研究理事会;

关键词：

Metapneumovirus; Vaccine; Virus-like particle; Neutralization; Antibody; NEUTRALIZING ANTIBODIES; PROTEIN; GLYCOPROTEIN; INDUCTION; VECTORS; DISEASE;

D O I：

10.1016/j.vaccine.2013.03.051

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Human metapneumovirus (HMPV) is a paramyxovirus that causes acute respiratory-tract infections in children and adults worldwide. A safe and effective vaccine could decrease the burden of disease associated with this novel pathogen. We engineered HMPV viral-like particles (HMPV-VLPs) derived from retroviral core particles that mimic the properties of the viral surface of two HMPV viruses of either lineage A or B. These VLPs functionally display F and G HMPV surface glycoproteins. When injected in mice, HMPV-VLPs induce strong humoral immune response against both homologous and heterologous strains. Moreover, the induced neutralizing antibodies prevented mortality upon subsequent infection of the lungs with both homologous and heterologous viruses. Upon challenge, viral titers in the lungs of immunized animals were significantly reduced as compared to those of control animals. In conclusion, a HMPV-VLP vaccine that induces cross-protective immunity in mice is a promising approach to prevent HMPV infections. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：2778 / 2785

页数：8

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