Label-free observation of DNA-hybridisation and endonuclease activity on a wave guide surface using a grating coupler

被引：30

作者：

Bier, FF

Scheller, FW

机构：

[1] Inst. Biochem. and Molec. Physiol., University of Potsdam, c/o Max-Delbrück-Centre, D-13125 Berlin

来源：

BIOSENSORS & BIOELECTRONICS | 1996年 / 11卷 / 6-7期

关键词：

DNA; oligonucleotides; hybridisation; gene probes; evanescent wave; grating coupler;

D O I：

10.1016/0956-5663(96)83300-8

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Hybridisation of nucleic acid oligomers to an immobilised target has been observed in real time using evanescent field technology. A biotinylated 24-mer with random sequence including the EcoRI recognition site was immobilised via streptavidin onto a grating coupler wave guide surface. Hybridisation of 22-mer, 15-mer and 8-mer was observed. Activity of restriction endonuclease EcoRI was visualised by measurement of the loss of bound DNA after incubation.

引用

页码：669 / 674

页数：6

共 22 条

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