H-NS Regulation of IraD and IraM Antiadaptors for Control of RpoS Degradation

被引：41

作者：

Battesti, A. ^{[1
]}

Tsegaye, Y. M. ^{[1
]}

Packer, D. G. ^{[1
]}

Majdalani, N. ^{[1
]}

Gottesman, S. ^{[1
]}

机构：

[1] NCI, Mol Biol Lab, Bethesda, MD 20892 USA

来源：

JOURNAL OF BACTERIOLOGY | 2012年 / 194卷 / 10期

关键词：

ESCHERICHIA-COLI; OSMOTIC REGULATION; MOLECULAR ADAPTER; RNA-POLYMERASE; SIGMA-FACTOR; PROTEIN; STPA; DNA; STABILITY; RSSB;

D O I：

10.1128/JB.00132-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

RpoS, the master sigma factor during stationary phase and under a variety of stress conditions, is regulated at multiple levels, including regulated degradation. Degradation is dependent upon ClpXP and the RssB adaptor protein. H-NS, a nucleoid-associated protein, affects the regulated degradation of RpoS; in the absence of H-NS, RpoS is stable. The mechanisms involved in this regulation were not known. We have found that H-NS inhibits the expression of iraD and iraM, the genes coding for two anti-adaptor proteins that stabilize RpoS when overexpressed. The regulation by H-NS of iraM is independent from the previously demonstrated regulation by the PhoP/PhoQ two-component system. Moreover, differences in the behavior of several hats alleles are explained by a role for StpA, an H-NS-like protein, in the regulation of RpoS stability. This finding parallels recent observations for a role of StpA in regulation of RpoS stability in Salmonella.

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页码：2470 / 2478

页数：9

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