Ion-pair liquid chromatographic determination of albendazole in cattle feed

A liquid chromatographic method is described for the determination of albendazole in cattle feeds. Samples are extracted with acetonitrile, and the extracts, after partitioning with hexane, are analyzed by ion-pair liquid chromatography with detection at 292 nm. Albendazole is eluted from the column well resolved from its oxidation byproducts. The method shows analytical characteristics well within acceptable limits. Recovery was in the range of 98.2-100.4%, while precision, expressed as percent relative standard deviation, in the range of 1.6-3.1%. Limit of detection was estimated at 0.5 mu g/g, and limit of determination at 1.0 mu g/g.