The tick protective antigen, 4D8, is a conserved protein involved in modulation of tick blood ingestion and reproduction

被引：150

作者：

de la Fuente, Jose

Almazan, Consuelo

Blas-Machado, Uriel

Naranjo, Victoria

Mangold, Atilio J.

Blouin, Edmour F.

Gortazar, Christian

Kocan, Katherine M.

机构：

[1] Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA

[2] UCLM, IREC, CSIC, JCCM, Ciudad Real 13071, Spain

[3] Oklahoma State Univ, Ctr Vet Hlth Sci, Diagnost Lab, Stillwater, OK 74078 USA

[4] Inst Nacl Tecnol Agropecuaria, Estac Expt Agropecuaria Rafaela, Rafaela, Santa Fe, Argentina

来源：

VACCINE | 2006年 / 24卷 / 19期

关键词：

tick vaccine; evolution; RNA interference; reproduction; subolesin;

D O I：

10.1016/j.vaccine.2006.02.046

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

The gene that encodes the tick protective antigen, 4D8, was cloned from 10 species belonging to 6 genera, and the nucleotide and amino acid sequences were analyzed. 4D8 nucleotide and protein sequences were conserved among these tick species with identity/similarity between 65-98 and 60-98%, respectively. The function of 4138 was characterized by RNA interference (RNAi) in five tick species. After the ticks were allowed to feed, degeneration of gut, salivary glands and reproductive tissues was observed, and tick survival, weight and oviposition were significantly reduced. 4D8 RNAi effected > 90% reduction in oviposition in all tick species tested. Because of the critical role that 4D8 plays during tick feeding and oviposition, which ultimately results in the reduction of tick progeny, we proposed the generic name "subolesin" (Latin, suboles: offspring, progeny) for tick 4D8 proteins and subA for the subolesin-encoding gene. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：4082 / 4095

页数：14

共 51 条

[1]

RNA interference: applicability in tick research [J].