A sequence-specific PCR marker linked with Pm21 distinguishes chromosomes 6AS, 6BS, 6DS of Triticum aestivum and 6VS of Haynaldia villosa

被引：31

作者：

Cao, A. Z.

Wang, X. E. ^{[1
]}

Chen, Y. P.

Zou, X. W.

Chen, P. D.

机构：

[1] Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Cytogenet Inst, Nanjing 210095, Jiangsu, Peoples R China

[2] Natl Engn Ctr Biochip Shanghai, Shanghai 201203, Peoples R China

来源：

PLANT BREEDING | 2006年 / 125卷 / 03期

关键词：

Haynaldia villosa; Triticum aestivum; homoeologous group 6 chromosomes; polymerase chain reaction marker; Pm21;

D O I：

10.1111/j.1439-0523.2006.01222.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

To develop markers linked with Pm21 located on chromosome 6VS of Haynaldia villosa, a pair of primers (NAU/xibao15F and NAU/xibao15R) were designed according to the sequence of a serine/threonine kinase gene (Contig17515), whose expression was induced by Blumeria graminis and selected from the gene expression experiment using the Barley GeneChip. Using genomic DNA of various genetic stocks including the wheat variety 'Yangmai#5', H. villosa, Triticum durum-H. villosa amphiploid, seven T. aestivum-H. villosa addition lines involving chromosomes 1V-7V, the translocation line T6VS.6AL, and 21 nullisomic-tetrasomic and eight deletion lines of T. aestivum 'Chinese Spring' as templates, four amplicons specific for 6VS, 6AS, 6BS and 6DS, respectively, were produced. F-2 individuals derived from the cross of 'Yangmai#5' x T6VS.6AL were analysed, and data indicate that NAU/xibao15(902) could be used as a co-dominant marker for selecting Pm21 located on 6VS.

引用

页码：201 / 205

页数：5

共 43 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[3] RESISTANCE TO POWDERY MILDEW IN WHEAT - A REVIEW OF ITS USE IN AGRICULTURE AND BREEDING PROGRAMS [J].

BENNETT, FGA .

PLANT PATHOLOGY, 1984, 33 (03) :279-300

[4] Identification of a microsatellite marker associated with Pm3 resistance alleles to powdery mildew in wheat [J].

Bougot, Y ;

Lemoine, J ;

Pavoine, MT ;

Barloy, D ;

Doussinault, G .

PLANT BREEDING, 2002, 121 (04) :325-329

[5] Identification of molecular markers linked to PM13, an Aegilops longissima gene conferring resistance to powdery mildew in wheat [J].

Cenci, A ;

D'Ovidio, R ;

Tanzarella, OA ;

Ceoloni, C ;

Porceddu, E .

THEORETICAL AND APPLIED GENETICS, 1999, 98 (3-4) :448-454

[6] The race-specific resistance gene to powdery mildew, MlRE, has a residual effect on adult plant resistance of winter wheat line RE714 [J].

Chantret, N ;

Pavoine, MT ;

Doussinault, G .

PHYTOPATHOLOGY, 1999, 89 (07) :533-539

[7] DEVELOPMENT AND MOLECULAR CYTOGENETIC ANALYSIS OF WHEAT-HAYNALDIA VILLOSA 6VS/6AL TRANSLOCATION LINES SPECIFYING RESISTANCE TO POWDERY MILDEW [J].

CHEN, PD ;

QI, LL ;

ZHOU, B ;

ZHANG, SZ ;

LIU, DJ .

THEORETICAL AND APPLIED GENETICS, 1995, 91 (6-7) :1125-1128

[8]

Duan XiaYu, 1998, Acta Phytophylacica Sinica, V25, P31

[9] The deletion stocks of common wheat [J].

Endo, TR ;

Gill, BS .

JOURNAL OF HEREDITY, 1996, 87 (04) :295-307

[10] IDENTIFICATION OF PROTEIN CODING REGIONS BY DATABASE SIMILARITY SEARCH [J].

GISH, W ;

STATES, DJ .

NATURE GENETICS, 1993, 3 (03) :266-272

← 1 2 3 4 5 →