A sequence-specific PCR marker linked with Pm21 distinguishes chromosomes 6AS, 6BS, 6DS of Triticum aestivum and 6VS of Haynaldia villosa

To develop markers linked with Pm21 located on chromosome 6VS of Haynaldia villosa, a pair of primers (NAU/xibao15F and NAU/xibao15R) were designed according to the sequence of a serine/threonine kinase gene (Contig17515), whose expression was induced by Blumeria graminis and selected from the gene expression experiment using the Barley GeneChip. Using genomic DNA of various genetic stocks including the wheat variety 'Yangmai#5', H. villosa, Triticum durum-H. villosa amphiploid, seven T. aestivum-H. villosa addition lines involving chromosomes 1V-7V, the translocation line T6VS.6AL, and 21 nullisomic-tetrasomic and eight deletion lines of T. aestivum 'Chinese Spring' as templates, four amplicons specific for 6VS, 6AS, 6BS and 6DS, respectively, were produced. F-2 individuals derived from the cross of 'Yangmai#5' x T6VS.6AL were analysed, and data indicate that NAU/xibao15(902) could be used as a co-dominant marker for selecting Pm21 located on 6VS.