Affinity surface-assisted laser desorption/ionization mass spectrometry for peptide enrichment

被引：22

作者：

Coffinier, Yannick ^{[1
]}

Nguyen, Nhung ^{[1
,2
]}

Drobecq, Herve ^{[3
]}

Melnyk, Oleg ^{[3
]}

Thomy, Vincent ^{[2
]}

Boukherroub, Rabah ^{[1
]}

机构：

[1] Univ Lille Nord France, IRI, CNRS, USR 3078, F-59658 Villeneuve Dascq, France

[2] Univ Lille Nord France, IEMN, UMR 8520, CNRS, F-59652 Villeneuve Dascq, France

[3] Univ Lille Nord France, IBL, CNRS, UMR 8161, F-59021 Lille, France

来源：

ANALYST | 2012年 / 137卷 / 23期

关键词：

DESORPTION-IONIZATION; POROUS SILICON; MATRIX; MONOLAYERS; NANOWIRE; NANOPARTICLES; ATTACHMENT; CHEMISTRY; PROTEINS; TARGET;

D O I：

10.1039/c2an35803d

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

In this paper, we report on the functionalization of silicon nanostructured (NanoSi) surface with an organic layer of nitrilotriacetic acid (NTA) and its subsequent use as an affinity surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) interface for histidine-tagged peptide enrichment and mass spectrometry analysis. The NTA terminal groups are immobilized onto the NanoSi surface via very stable Si-C covalent bonds. The NTA-modified NanoSi (NTA-NanoSi) interface was characterized by contact angle measurements, Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The NTA-NanoSi interface has shown a good selectivity toward His-tagged peptide and permits its enrichment from an artificial mixture of both tagged and untagged peptides and its subsequent mass spectrometry detection with good signal/noise ratio.

引用

收藏

页码：5527 / 5532

页数：6

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