In Vitro Cultivation of the Hymenoptera Genetic Model, Nasonia

被引：16

作者：

Brucker, Robert M. ^{[1
]}

Bordenstein, Seth R. ^{[1
,2
]}

机构：

[1] Vanderbilt Univ, Dept Biol Sci, Nashville, TN 37235 USA

[2] Vanderbilt Univ, Dept Pathol Microbiol & Immunol, Nashville, TN USA

来源：

PLOS ONE | 2012年 / 7卷 / 12期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1371/journal.pone.0051269

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The wasp genus Nasonia is a genetic model with unique advantages for the study of interspecific differences, including haplodiploidy and interfertile species. However, as a parasitoid, Nasonia is confined within a fly host, thus restricting direct observations and manipulation of development over time. Here, we present the first in vitro cultivation method for this system that decouples Nasonia from its host, allowing continuous observations from embryo to adulthood. Using transwell plates and a simple Nasonia rearing medium, we demonstrate a technique that will significantly expand the utility of the Nasonia model.

引用

页数：5

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