Biological and sequence comparisons of Potato virus Y isolates associated with potato tuber necrotic ringspot disease

被引:64
作者
Boonham, N [1 ]
Walsh, K [1 ]
Hims, M [1 ]
Preston, S [1 ]
North, J [1 ]
Barker, I [1 ]
机构
[1] Cent Sci Lab, York YO41 1LZ, N Yorkshire, England
关键词
potyvirus; PTNRD; PVY; recombination;
D O I
10.1046/j.1365-3059.2002.00681.x
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The biological and molecular relationships between a large number of Potato virus Y (PVY) isolates were examined, concentrating mainly on isolates associated with potato tuber necrotic ringspot disease (PTNRD). Following detailed analysis of the coat-protein gene, four main groups were identified which broadly corresponded to the phenotype of the different isolates. The groups comprised the ordinary strain (PVYO), the necrotic strain (PVYN), the C strain (PVY) and a group of recombinant (between ordinary and necrotic) isolates. In the latter group, all members were associated with PTNRD. However, four nonrecombinant isolates were also identified which were associated with PTNRD or tuber necrosis. Three were from tubers showing PTNRD symptoms in the field, while the fourth originated from symptomless tubers, but could cause necrotic rings on tubers under glasshouse conditions. The results show that although coat-protein recombination is always found associated with the PTNRD phenotype, some nonrecombinant isolates have very similar biological properties.
引用
收藏
页码:117 / 126
页数:10
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