CDP-choline treatment induces brain plasticity markers expression in experimental animal stroke

被引:57
作者
Gutierrez-Fernandez, Maria [2 ]
Rodriguez-Frutos, Berta [2 ]
Fuentes, Blanca [1 ]
Teresa Vallejo-Cremades, Maria [2 ]
Alvarez-Grech, Julia [2 ]
Exposito-Alcaide, Mercedes [2 ]
Diez-Tejedor, Exuperio [1 ,2 ]
机构
[1] Univ Autonoma Madrid, La Paz Univ Hosp, Dept Neurol, Stroke Ctr,Neurosci Area IdiPAZ,Hlth Res Inst, Madrid 28046, Spain
[2] Univ Autonoma Madrid, La Paz Univ Hosp, Neurosci & Cerebrovasc Res Lab, Neurosci Area IdiPAZ,Hlth Res Inst, Madrid 28046, Spain
关键词
CDP-choline; Recovery; Brain plasticity markers; Stroke; Rats; EXPERIMENTAL FOCAL ISCHEMIA; CEREBRAL-ARTERY OCCLUSION; HIPPOCAMPAL-NEURONS; NEUROVASCULAR UNIT; INFARCT VOLUME; GROWTH-FACTOR; CITICOLINE; RAT; ANGIOGENESIS; NEUROPROTECTION;
D O I
10.1016/j.neuint.2011.12.015
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We investigated the effect of CDP-choline on brain plasticity markers expression in the acute phase of cerebral infarct in an experimental animal model. Male Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (pMCAO) and treated or not with CDP-choline (500 mg/kg) daily for 14 days starting 30 min after pMCAO. Functional status was evaluated with Roger's test; lesion volume with magnetic resonance imaging (MRI) and hematoxylin and eosin staining (H & E); cell death with TUNEL; cellular proliferation with BrdU immunohistochemistry; vascular endothelial growth factor (VEGF), synaptophysin, glial fibrillary acidic protein (GFAP) and low-density lipoprotein receptor-related protein (LRP) by immunofluorescence and Western-blot techniques. CDP-choline significantly improved functional recovery and decreased lesion volume on MRI, TUNEL-positive cell number and LRP levels at 14 days. In addition, CDP-choline significantly increased BrdU, VEGF and synaptophysin values and decreased GFAP levels in the peri-infarct zone compared with the infarct group. In conclusion, our data indicate that CDP-choline improved functional recovery after permanent middle cerebral artery occlusion in association with reductions in lesion volume, cell death and LRP expression. In fact, CDP-choline increased cell proliferation, vasculogenesis and synaptophysin levels and reduced GFAP levels in the peri-infarct area of the ischemic stroke. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:310 / 317
页数:8
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