Function, Targets, and Evolution of Caenorhabditis elegans piRNAs

被引：261

作者：

Bagijn, Marloes P. ^{[1
,2
]}

Goldstein, Leonard D. ^{[1
,2
]}

Sapetschnig, Alexandra ^{[1
,2
]}

Weick, Eva-Maria ^{[1
,2
]}

Bouasker, Samir ^{[3
]}

Lehrbach, Nicolas J. ^{[1
,2
]}

Simard, Martin J. ^{[3
]}

Miska, Eric A. ^{[1
,2
]}

机构：

[1] Univ Cambridge, Gurdon Inst, Cambridge CB2 1QN, England

[2] Univ Cambridge, Dept Biochem, Cambridge CB2 1QN, England

[3] Univ Laval, Canc Res Ctr, Hotel Dieu Quebec CHUQ, Quebec City, PQ G1R 2J6, Canada

来源：

SCIENCE | 2012年 / 337卷 / 6094期

基金：

英国惠康基金; 加拿大健康研究院;

关键词：

C-ELEGANS; DNA METHYLATION; PIWI; RNA; DROSOPHILA; 21U-RNAS; PATHWAY; AMPLIFICATION; ARGONAUTE; REVEALS;

D O I：

10.1126/science.1220952

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Piwi-interacting RNAs (piRNAs) are small RNAs required to maintain germline integrity and fertility, but their mechanism of action is poorly understood. Here we demonstrate that Caenorhabditis elegans piRNAs silence transcripts in trans through imperfectly complementary sites. Target silencing is independent of Piwi endonuclease activity or "slicing." Instead, piRNAs initiate a localized secondary endogenous small interfering RNA (endo-siRNA) response. Endogenous protein-coding gene and transposon transcripts exhibit Piwi-dependent endo-siRNAs at sites complementary to piRNAs and are derepressed in Piwi mutants. Genomic loci of piRNA biogenesis are depleted of protein-coding genes and tend to overlap the start and end of transposons in sense and antisense, respectively. Our data suggest that nematode piRNA clusters are evolving to generate piRNAs against active mobile elements. Thus, piRNAs provide heritable, sequence-specific triggers for RNA interference in C. elegans.

引用

页码：574 / 578

页数：5

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