Screening of various glycosidases for the synthesis of octyl glucoside

被引：53

作者：

Ducret, A ^{[1
]}

Trani, M ^{[1
]}

Lortie, R ^{[1
]}

机构：

[1] Natl Res Council Canada, Biotechnol Res Inst, Microbial & Enzyme Technol Grp, Bioproc Sector, Montreal, PQ H4P 2R2, Canada

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2002年 / 77卷 / 07期

关键词：

beta-glucosidase; screening; alkylglycosides; reverse hydrolysis; transglycosylation; water activity organic solvent;

D O I：

10.1002/bit.10156

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Thirteen glycosidases of microbial origin and almond beta-glycosidase were assayed in octanol/DMF (80:20, v/v), using a combination of hydrolysis, transglycosylation, and condensation reactions, in order to assess their potential for the production of alkyl glucosides. The two mesophile enzymes were highly impaired by the organic media. Three of the 11 thermophile enzymes gave interesting results in the hydrolysis and transglycosylation reactions, but they were highly inhibited by glucose. This made their use in a condensation reaction less interesting than the use of almond beta-glucosidase, which has a lower activity but shows less inhibition by the glucose. (C) 2002 Government of Canada. Exclusive worldwide publication rights in this article have been transferred to John Wiley Sons, Inc.

引用

页码：752 / 757

页数：6

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