Enzymatic synthesis of dodecyl.beta-D-glucopyranoside catalyzed by Candida molischiana 35M5N beta-glucosidase

被引：3

作者：

Gueguen, Y ^{[1
]}

Chemardin, P ^{[1
]}

Pommares, P ^{[1
]}

Arnaud, A ^{[1
]}

Galzy, P ^{[1
]}

机构：

[1] ECOLE NATL SUPER AGRON MONTIPELLIER,CHAIR MICROBIOL IND & GENET MICOORGANISMES,F-34060 MONTPELLIER,FRANCE

来源：

BIORESOURCE TECHNOLOGY | 1995年 / 53卷 / 03期

关键词：

Dodecyl.beta-D-glucopyranoside; beta-glucosidase; condensation reaction; transglucosylation;

D O I：

10.1016/0960-8524(95)00095-2

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The enzymatic synthesis of dodecyl.beta-D-glucopyrano-side was studied by using the beta-glucosidase from Candida molischiana 35M5N in the examination of transglucosylation and hydrolysis reversion reactions. On account of cost considerations the condensation reaction was optimized. The effects of the extraction solvent, the initial 1-dodecanol and glucose concentrations, the temperature and the incubation time were studied. The optimal conditions for the enzymatic synthesis of dodecyl.beta-D-glucopyranoside by the condensation reaction were: 90% (v/v) 1-dodecanol initial concentration; glucose concentration (50 mM), temperature 40 degrees C and reaction time 20 h.

引用

页码：263 / 267

页数：5

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