GLUCOSIDE SYNTHESIS BY GLUCOAMYLASE OR BETA-GLUCOSIDASE IN ORGANIC-SOLVENTS

被引：43

作者：

LAROUTE, V ^{[1
]}

WILLEMOT, RM ^{[1
]}

机构：

[1] INST NATL SCI APPL,DEPT GENIE BIOCHIM & ALIMENTAIRE,CNRS,UA 544,AVE RANGUEIL,F-31077 TOULOUSE,FRANCE

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 03期

关键词：

D O I：

10.1007/BF01023353

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glucoamylase and beta-glucosidase have been shown to catalyse respectively alpha- or beta-glucoside synthesis in water-organic mixtures. The effects of water content, water activity, organic solvent nature and substrate concentration on the reaction yield were studied. Maximum yields were obtained with 10% (v/v) of water in primary alcohol systems and 15% in diol systems. Beta-glucosidase has given the best yields. This enzyme has presented for alcohols a higher affinity than glucoamylase. A difference of stereospecificity was observed with the two enzymes and diols.

引用

页码：169 / 174

页数：6

共 9 条

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