An observation of unusual temperature effects for enantioselective CZE employing highly sulfated-β-cyclodextrin

被引：8

作者：

Westall, A ^{[1
]}

Mallmström, T ^{[1
]}

Petersson, P ^{[1
]}

机构：

[1] AstraZeneca R&D Lund, Pharmaceut & Analyt R&D, Analyt Dev PAR&D, S-22187 Lund, Sweden

来源：

ELECTROPHORESIS | 2006年 / 27卷 / 04期

关键词：

capillary zone electrophoresis; cyclodextrins; enantioselectivity; temperature;

D O I：

10.1002/elps.200410284

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper data are presented suggesting that for enantiomers of basic and neutral analytes, the concentrations of highly sulfated CDs typically used are above the optimum since lower concentrations result in unacceptable migration times. This situation arises because of the high binding affinities often obtained with sulphated CDs. In a system being over the optimum CID concentration increasing the temperature of the capillary offers a capability to improve selectivity and reduce migration times.

引用

页码：859 / 864

页数：6

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