Detection of attomolar concentrations of alkaline phosphatase by capillary electrophoresis using laser-induced fluorescence detection

被引：62

作者：

Craig, DB ^{[1
]}

Wong, JCY ^{[1
]}

Dovichi, NJ ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON,AB T6G 2G2,CANADA

来源：

ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 04期

关键词：

D O I：

10.1021/ac950650z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Alkaline phosphatase can be assayed by: monitoring the conversion of the fluorogenic substrate AttoPhos into the highly fluorescent product AttoFluor, We have used capillary electrophoresis with laser-induced fluorescence detection to monitor this reaction, The concentration limit of detection (3 sigma) of alkaline phosphatase is 1.5 x 10(-17) M (2.1 fg/mL), which corresponds to a mass limit of detection of nine molecules (1.5 x 10(-23) mol) contained within a 1-mu L sample volume.

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页码：697 / 700

页数：4

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