Ultrahigh resolution in proton solid-state NMR spectroscopy at high levels of deuteration

被引：251

作者：

Chevelkov, V ^{[1
]}

Rehbein, K ^{[1
]}

Diehl, A ^{[1
]}

Reif, B ^{[1
]}

机构：

[1] Leibniz Inst Mol Pharmakol FMP, D-13125 Berlin, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 23期

关键词：

line widths; magic-angle spinning; predeuteration; proteins; solid-state NMR spectroscopy; structure elucidation;

D O I：

10.1002/anie.200600328

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Slim peaks: Using a perdeuterated protein recrystallized from a 10:90 H2O:D2O mixture in magic-angle spinning (MAS) solid-state NMR spectroscopy experiments gives small 1H line widths at moderate spinning frequencies without application of homonuclear decoupling. This labeling strategy opens new perspectives for assignment of large protein spin systems. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：3878 / 3881

页数：4

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