Histone acetylation regulates p21WAF1 expression in human colon cancer cell lines

被引：39

作者：

Chen, Ying-Xuan ^{[1
]}

Fang, Jing-Yuan ^{[1
]}

Zhu, Hong-Yin ^{[1
]}

Lu, Rong ^{[1
]}

Cheng, Zhong-Hua ^{[1
]}

Qiu, De-Kai ^{[1
]}

机构：

[1] Shanghai Med Univ 2, Shanghai Inst Digest Dis, Renji Hosp, Shanghai 200001, Peoples R China

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2004年 / 10卷 / 18期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.3748/wjg.v10.i18.2643

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To investigate the effect of histone acetylation on regulation of p21(WAF1) gene expression in human colon cancer cell lines. METHODS: Two cell lines, Colo-320 and SW1116 were treated with either trichostatin or sodium butyrate. Expressions of p21(WAF1) mRNA and protein were detected by real-time RT-PCR and Western blotting, respectively. Acetylation of two regions of p21(WAF1) gene-associated histones and total cellular histones were examined by chromatin immunoprecipitation assay and Western blotting. RESULTS: Trichostatin or sodium butyrate re-activated p21(WAF1) transcription resulted in up-regulated p21(WAF1) protein level in colon cancer cell lines. Those effects were accompanied by an accumulation of acetylated histones in total cellular chromatin and p21(WAF1) gene-associated region of chromatin. CONCLUSION: Histone acetylation regulates p21(WAF1) expression in human colon cancer cell lines, Colo-320 and SW1116.

引用

收藏

页码：2643 / 2646

页数：4

相关论文

共 25 条

[1]

Histone acetylation and cancer [J].

Archer, SY ;

Hodin, RA .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 1999, 9 (02) :171-174

[2]

Developing robust frequentist and Bayesian fish stock assessment methods [J].

Chen, Y ;

Jiao, Y ;

Chen, LQ .

FISH AND FISHERIES, 2003, 4 (02) :105-120

[3]

FUNCTIONAL-ANALYSIS OF THE TRANSFORMING GROWTH-FACTOR-BETA RESPONSIVE ELEMENTS IN THE WAF1/CIP1/P21 PROMOTER [J].

DATTO, MB ;

YU, Y ;

WANG, XF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (48) :28623-28628

[4]

Gene expression array of HTLV type 1-infected T cells: Up-regulation of transcription factors and cell cycle genes [J].

De la Fuente, C ;

Deng, LW ;

Santiago, F ;

Arce, L ;

Wang, L ;

Kashanchi, F .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 2000, 16 (16) :1695-1700

[5]

P53-DEPENDENT INHIBITION OF CYCLIN-DEPENDENT KINASE-ACTIVITIES IN HUMAN FIBROBLASTS DURING RADIATION-INDUCED G1 ARREST [J].

DULIC, V ;

KAUFMANN, WK ;

WILSON, SJ ;

TLSTY, TD ;

LEES, E ;

HARPER, JW ;

ELLEDGE, SJ ;

REED, SI .

CELL, 1994, 76 (06) :1013-1023

[6]

Effects of histone acetylation and DNA methylation on p21WAF1 regulation [J].

Fang, JY ;

Lu, YY .

WORLD JOURNAL OF GASTROENTEROLOGY, 2002, 8 (03) :400-405

[7]

Histone acetylation in chromatin structure and transcription [J].

Grunstein, M .

NATURE, 1997, 389 (6649) :349-352

[8]

Transcriptional activation of the enterocyte differentiation marker intestinal alkaline phosphatase is associated with changes in the acetylation state of histone H3 at a specific site within its promoter region in vitro [J].

Hinnebusch, BF ;

Henderson, JW ;

Siddique, A ;

Malo, MS ;

Zhang, WY ;

Abedrapo, MA ;

Hodin, RA .

JOURNAL OF GASTROINTESTINAL SURGERY, 2003, 7 (02) :237-244

[9]

The effects of short-chain fatty acids on human colon cancer cell phenotype are associated with histone hyperacetylation [J].

Hinnebusch, BF ;

Meng, SF ;

Wu, JT ;

Archer, SY ;

Hodin, RA .

JOURNAL OF NUTRITION, 2002, 132 (05) :1012-1017

[10]

Transcriptional activation of p21WAF1/C1P1 by apicidin, a novel histone deacetylase inhibitor [J].

Kim, JS ;

Lee, SM ;

Lee, T ;

Lee, YW ;

Trepel, JB .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 281 (04) :866-871