Use of surface-modified capillaries in the separation and characterization of metallothionein isoforms by capillary electrophoresis inductively coupled plasma mass spectrometry

被引：42

作者：

Wang, ZX ^{[1
]}

Prange, A ^{[1
]}

机构：

[1] Inst Coastal Res Phys & Chem Anal, GKSS Res Ctr, D-21502 Geesthacht, Germany

来源：

ANALYTICAL CHEMISTRY | 2002年 / 74卷 / 03期

关键词：

D O I：

10.1021/ac010862y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The separation of metallothionein (MT) isoforms using capillary electrophoresis (CE) has been improved by applying surface-modified capillaries, and the metal composition of MTs has been characterized by subsequent inductively coupled plasma sector field mass spectrometty (ICPSFMS). Nine MT complexes in a commercial preparation from rabbit fiver were successfully separated on an anionic polymer-coated column, prepared by immobilizing poly(2-acrylamido-2-methyl-1-propanesulfonic acid) on the fused-silica surface via a linking agent. On uncoated capillaries or those coated dynamically with cationic materials, only three complexes could be separated. Online isotope dilution analysis combined with CE/ICPSFMS indicated the stoichiometric molar metal contents in the MT complexes.

引用

页码：626 / 631

页数：6

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