A multicopper oxidase gene from Candida albicans:: cloning, characterization and disruption

被引：88

作者：

Eck, R ^{[1
]}

Hundt, S ^{[1
]}

Härtl, A ^{[1
]}

Roemer, E ^{[1
]}

Künkel, W ^{[1
]}

机构：

[1] Hans Knoll Inst Nat Stoff Forsch EV, Abt Mikrobielle Infekt Biol, D-07745 Jena, Germany

来源：

MICROBIOLOGY-UK | 1999年 / 145卷

关键词：

multicopper oxidase; Candida albicans; gene disruption; pathogenicity; iron uptake;

D O I：

10.1099/00221287-145-9-2415

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A multicopper oxidase gene from the human pathogenic yeast Candida albicans was isolated and characterized. An open reading frame of 1872 bp. designated CaFET3, was identified, encoding a predicted protein of 624 amino acids and a molecular mass of 70.5 kDa. The identity between the deduced amino acid sequences of CaFET3 and the Saccharomyces cerevisiae FET3 gene is 55 %. CaFET3 was localized on chromosome 6. A null mutant (fet3D/fet3D) was constructed by sequential gene disruption. Unlike the C. albicans SC5314 wildtype strain the fef3D mutant was unable to grow in low-iron medium. The lack of growth of a S. cerevisiae fet3D mutant in iron-limited medium was compensated by transformation with CaFET3. The null mutant strain showed no change in pathogenicity compared with the wild-type strain in the mouse model of systemic candidiasis.

引用

页码：2415 / 2422

页数：8

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