Phylogenetic analysis of Portuguese bovine viral diarrhoea virus

被引：32

作者：

Barros, Silvia C.

Ramos, Fernanda

Pauperio, Sonia

Thompson, Gertrude

Fevereiro, Miguel ^{[1
]}

机构：

[1] Lab Nacl Invest Vet, Dept Virol, Lisbon, Portugal

[2] Univ Porto, Inst Ciencias Biomed Abel Salazar, Dept Clin, Oporto, Portugal

来源：

VIRUS RESEARCH | 2006年 / 118卷 / 1-2期

关键词：

BVDV-1; BVDV-2; Pestivirus; phylogenetic analysis;

D O I：

10.1016/j.virusres.2005.12.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bovine viral diarrhoea virus (BVDV) infections are an important cause of morbidity and mortality worldwide in dairy and beef cattle. To date, little is know about BVDV genotypes circulating in Portugal. For this purpose, a fragment within the 5'-untranslated region (5'-UTR) from 34 Portuguese field strains of BVDV was amplified by RT-PCR, cloned and sequenced. A maximum-likelihood phylogenetic analysis revealed that most of viruses, originated from cattle from different regions of the country, belong to BVDV type 1 (BVDV-1), genotypes 1b (n = 19), la (n = 6), 1d (n = 3) and le (n = 3); whereas three viruses clustered in BVDV type 2 (BVDV-2). The results from this study demonstrate that BVDV-1b is the most prevalent genotype and also shows the presence of BVDV-2 in Portugal. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：192 / 195

页数：4

共 33 条

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