Agonists of proteinase-activated receptor 2 excite guinea pig ileal myenteric neurons

被引:33
作者
Linden, DR
Manning, BP
Bunnett, NW
Mawe, GM
机构
[1] Univ Vermont, Coll Med, Dept Anat & Neurobiol, Burlington, VT 05405 USA
[2] Univ Calif San Francisco, Dept Surg, San Francisco, CA 94143 USA
[3] Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94143 USA
关键词
euteric nervous system; neuroimmune interaction; electrophysiology; thrombin receptors; trypsin;
D O I
10.1016/S0014-2999(01)01447-9
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The effects of proteinase-activated receptor 2 (PAR2) agonists on the electrical properties of intact guinea pig ileal myenteric neurons were measured with intracellular microelectrodes. Approximately 52% of AH neurons and 41% of S neurons responded to pressure ejection of SLIGRL-NH2 or trypsin with a prolonged depolarization that was often accompanied by increased excitability. When added to the bathing solution, trypsin caused a concentration-dependent depolarization of responding neurons with an estimated EC50 value of 87 nM. Collectively, these novel observations indicate that PAR2 excites a proportion of myenteric neurons, which may contribute to dysmotility during intestinal inflammation. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:311 / 314
页数:4
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