Colonic and jejunal motor disturbances after colonic antigen challenge of sensitized rat

被引:17
作者
Oliver, MR
Tan, DMTM
Kirk, DR
Rioux, KP
Scott, RB
机构
[1] UNIV CALGARY,HLTH SCI CTR,DEPT PEDIAT,CALGARY,AB T2N 4N1,CANADA
[2] UNIV CALGARY,GASTROINTESTINAL RES GRP,CALGARY,AB,CANADA
关键词
D O I
10.1053/gast.1997.v112.pm9178692
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background & Aims: Inflammation in the colon may alter motility in the proximal gut and potentiate clinical symptoms, The aim of this study was to characterize the effect of colonic anaphylaxis on local (colonic) and remote (small intestinal) motility and identify the mechanism and mediators involved. Methods: Rats were sensitized by intraperitoneal injection of 10 mu g egg albumin and surgically prepared with electrodes in jejunum and colon and a colostomy tube. Colonic and jejunal myoelectric activity were recorded in fasted animals before and after colonic antigen challenge without and then after pretreatment with specific antagonists, Results: Colonic antigen challenge of sensitized rats was associated with significant (1) increase in colonic myoelectric spike activity, (2) disruption of fasting jejunal motility and initiation of aborally propagating spike complexes, and (3) increase in plasma rat mast cell protease II levels with a decrease in granulated mast cells in colon but not jejunum, The myoelectric disturbance in both colon and jejunum was inhibited significantly by pretreatment with atropine and hexamethonium, doxantrazole, cyclooxygenase, and lipoxygenase inhibitors. Methysergide inhibited only the jejunal disturbance, Conclusions: Colonic antigen challenge of sensitized animals results in local mast cell activation and the release of mediators that modulate neural pathways to initiate both a local colonic and a remote jejunal myoelectric disturbance.
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页码:1996 / 2005
页数:10
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