Production of enzymes by Alteromonas sp A321 to degrade polysaccharides from Enteromorpha prolifera

被引：51

作者：

Li, Yinping ^{[1
]}

Wang, Jin ^{[1
]}

Yu, Yuan ^{[1
]}

Li, Xiang ^{[1
]}

Jiang, Xiaolu ^{[1
]}

Hwang, Hueymin ^{[2
]}

Wang, Peng ^{[1
]}

机构：

[1] Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

[2] Jackson State Univ, Dept Biol, Jackson, MS 39217 USA

来源：

CARBOHYDRATE POLYMERS | 2013年 / 98卷 / 01期

基金：

美国国家科学基金会;

关键词：

Polysaccharides from Enteromorpha prolifera; Degradase; Alteromonas sp A321; Identification; Enzymatic hydrolysis; Response surface methodology; RESPONSE-SURFACE METHODOLOGY; SULFATED POLYSACCHARIDES; MEDIUM COMPONENTS; IN-VITRO; OPTIMIZATION; OLIGOSACCHARIDES; FERMENTATION; PURIFICATION; PROTEASE; XYLANASE;

D O I：

10.1016/j.carbpol.2013.07.016

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Polysaccharides from Enteromorpha prolifera (PE) are becoming increasingly popular due to its bioactivity and abundant source. Screening novel microorganisms which could secrete enzymes to degrade PE efficiently for oligosaccharides production is a promising solution to improve its application. In this study, a marine bacterium that can produce enzymes to degrade PE specifically was selected. It was identified as Alteromonas sp. A321, based on the biochemical properties and 16S rDNA gene sequencing. In order to maximize the activity of degradase for polysaccharides from E. prolifera COPE), the effects of medium composition and culture conditions were investigated. The highest DPE production was obtained in the medium consisting of K2HPO4 0.15%, PE 0.9%, NaNO3 0.4%, NaCl 1.0% and MgSO4 0.05%. The degradase activity was enhanced from original 0.391 U/ml to 0.744 U/ml. DPE show high efficiency and substrate specificity to PE with 63.53% of reducing sugar production in the 7 h hydrolysis. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.

引用

页码：988 / 994

页数：7

共 28 条

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