Determination of the animal origin of raw food by species-specific PCR

被引:60
作者
Herman, L [1 ]
机构
[1] Minist Small Enterprises TRaders & Agr Belgium, Agr Res Ctr, Dept Anim Prod Qual, B-9090 Melle, Belgium
关键词
PCR cytochrome b; milk; animal identification;
D O I
10.1017/S0022029901004940
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Specific PCR, amplifying a fragment of the mitochondrially encoded cytochrome b gene, are developed for discrimination of chicken, turkey, pig, cow and sheep. these PCR tests could be applied for detection and discrimination of animal food ingredients. For origin identification of milk, the bovine PCR is applied on DNA extracted with the DNeasy(TM) Tissue kit. A PCR of 30 cycles reached a sensitivity of 720 somatic cells in the PCR reaction corresponding to 2.9 x 10(4) cells per ml milk. A PCR of 40 cycles could detect 0.72 to 0.144 somatic cells in the PCR reaction corresponding to 29 to 2.9 cells per ml of milk.
引用
收藏
页码:429 / 436
页数:8
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