Relative Strengths of the Class 1 Integron Promoter Hybrid 2 and the Combinations of Strong and Hybrid 1 with an Active P2 Promoter

被引：40

作者：

Papagiannitsis, Costas C. ^{[1
]}

Tzouvelekis, Leonidas S. ^{[2
]}

Miriagou, Vivi ^{[1
]}

机构：

[1] Hellenic Pasteur Inst, Bacteriol Lab, Athens 11521, Greece

[2] Univ Athens, Sch Med, Dept Microbiol, GR-11527 Athens, Greece

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2009年 / 53卷 / 01期

关键词：

ANTIBIOTIC-RESISTANCE GENES; METALLO-BETA-LACTAMASE; KLEBSIELLA-PNEUMONIAE; PLASMID; CASSETTES; SEQUENCE;

D O I：

10.1128/AAC.00912-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The relative strengths of the uncommon promoters hybrid 2, hybrid 1 with an active P2 promoter (hybrid 1 + P2), and strong + P2, which drive transcription of resistance genes in class 1 integrons, were evaluated using bla(GES-1) as a reporter gene cassette. Hybrid 2 was stronger than hybrid 1. Coupling P2 with the strong promoter and with hybrid 1 caused a measurable increase in GES-1 expression.

引用

页码：277 / 280

页数：4

共 15 条

[1]

[Anonymous], 1989, Molecular Cloning: A Laboratory Manual

[2] NEW MOBILE GENE CASSETTES CONTAINING AN AMINOGLYCOSIDE RESISTANCE GENE, AACA7, AND A CHLORAMPHENICOL RESISTANCE GENE, CATB3, IN AN INTEGRON IN PBWH301 [J].

BUNNY, KL ;

HALL, RM ;

STOKES, HW .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (03) :686-693

[3] Characterization of the IncA/C plasmid pCC416 encoding VIM-4 and CMY-4 β-lactamases [J].

Colinon, Celine ;

Miriagou, Vivi ;

Carattoli, Alessandra ;

Luzzaro, Francesco ;

Rossolini, Gian Maria .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2007, 60 (02) :258-262

[4] EXPRESSION OF ANTIBIOTIC-RESISTANCE GENES IN THE INTEGRATED CASSETTES OF INTEGRONS [J].

COLLIS, CM ;

HALL, RM .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (01) :155-162

[5] Characterization of a novel plasmid-mediated cephalosporinase (CMY-9) and its genetic environment in an Escherichia coli clinical isolate [J].

Doi, Y ;

Shibata, N ;

Shibayama, K ;

Kamachi, K ;

Kurokawa, H ;

Yokoyama, K ;

Yagi, T ;

Arakawa, Y .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (08) :2427-2434

[6] Genetic basis for dissemination of armA [J].

González-Zorn, B ;

Catalan, A ;

Escudero, JA ;

Teshager, T ;

Porrero, C ;

Moreno, MA .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2005, 56 (03) :583-585

[7] Molecular evolution of metallo-β-lactamase-producing Pseudomonas aeruginosa in a nosocomial setting of high-level endemicity [J].

Lagatolla, Cristina ;

Edalucci, Elisabetta ;

Dolzani, Lucilla ;

Riccio, Maria Letizia ;

De Luca, Filomena ;

Medessi, Erica ;

Rossolini, Gian Maria ;

Tonin, Enrico Angelo .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (07) :2348-2353

[8] DIVERSITY AND RELATIVE STRENGTH OF TANDEM PROMOTERS FOR THE ANTIBIOTIC-RESISTANCE GENES OF SEVERAL INTEGRONS [J].

LEVESQUE, C ;

BRASSARD, S ;

LAPOINTE, J ;

ROY, PH .

GENE, 1994, 142 (01) :49-54

[9] MOLECULAR-CLONING AND NUCLEOTIDE SEQUENCING OF A NOVEL AMINOGLYCOSIDE 6'-N-ACETYLTRANSFERASE GENE FROM AN R-PLASMID OF SALMONELLA-TYPHIMURIUM S24 ISOLATED IN TAIWAN [J].

PENG, CF ;

CHANG, SF .

MICROBIOLOGY AND IMMUNOLOGY, 1992, 36 (04) :339-350

[10] Biochemical sequence analyses of GES-1, a novel class A extended-spectrum β-lactamase, and the class 1 integron In52 from Klebsiella pneumoniae [J].

Poirel, L ;

Le Thomas, I ;

Naas, T ;

Karim, A ;

Nordmann, P .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (03) :622-632

← 1 2 →