Letter: The use of electrospray ionization mass spectrometry to distinguish the lot-to-lot heterogeneity of an antigen specific monoclonal antibody from a specific cellular clone

被引：13

作者：

Adamczyk, M ^{[1
]}

Gebler, JC ^{[1
]}

Harrington, CA ^{[1
]}

Sequeira, AF ^{[1
]}

机构：

[1] Abbott Labs, Dept Chem 9NM, Div Diagnost, Abbott Pk, IL 60064 USA

来源：

EUROPEAN MASS SPECTROMETRY | 1999年 / 5卷 / 03期

关键词：

electrospray ionization; monoclonal antibodies; fingerprinting;

D O I：

10.1255/ejms.264

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

Liquid chromatography/electrospray ionization mass spectrometry was used to investigate eight different preparations of an antithyroid stimulating hormone murine monoclonal antibody, obtained from a single cellular clone grown under identical conditions. While the spectra of the intact IgG provided a primary fingerprint distinguishing the investigated lots, subsequent analysis of the antibody fragments further confirmed the differences.

引用

页码：165 / 168

页数：4

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