Immunoassay reagents for thyroid testing .2. Binding properties and energetic parameters of a T-4 monoclonal antibody and its fab fragment with a library of thyroxine analog biosensors using surface plasmon resonance

被引：26

作者：

Adamczyk, M

Gebler, JC

Gunasekera, AH

Mattingly, PG

Pan, Y

机构：

[1] Diagnostics Division, Abbott Laboratories, Building AP 20, Abbott Park, IL 60064

来源：

BIOCONJUGATE CHEMISTRY | 1997年 / 8卷 / 02期

关键词：

D O I：

10.1021/bc960088z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A library of 16 thyroxine analog biosensors were prepared for use on the BIAcore surface plasmon resonance instrument. An anti-thyroxine monoclonal antibody and its Fab fragment were tested with each biosensor to generate the association and dissociation rate constants, equilibrium association constants, and Gibbs free energy values for characterizing the binding event.

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页码：133 / 145

页数：13

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