IL-12-Dependent Inducible Expression of the CD94/NKG2A Inhibitory Receptor Regulates CD94/NKG2C+ NK Cell Function

被引:54
作者
Saez-Borderias, Andrea [1 ]
Romo, Neus [1 ]
Magri, Giuliana [1 ]
Guma, Monica [1 ]
Angulo, Ana [2 ]
Lopez-Botet, Miguel [1 ,3 ]
机构
[1] Univ Pompeu Fabra, Mol Immunopathol Unit, Barcelona 08003, Spain
[2] Inst Invest Biomed August Pi & Sunyer, Barcelona, Spain
[3] Hosp del Mar, Inst Municipal Invest Med, Barcelona, Spain
关键词
NATURAL-KILLER-CELLS; HLA CLASS-I; HUMAN CYTOMEGALOVIRUS-INFECTION; HUMAN T-LYMPHOCYTES; DENDRITIC CELLS; ACTIVATING CD94/NKG2C; SURFACE EXPRESSION; MOLECULE QA-1(B); RECOGNITION; ANTIGEN;
D O I
10.4049/jimmunol.182.2.829
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The inhibitor), CD94/NKG2A and activating CD94/NKG2C killer lectin-like receptors specific for HLA-E have been reported to be selectively expressed by discrete NK and T cell subsets. In the present study, minor proportions of NK and T cells coexpressing both CD94/NKG2A and CD94/NKG2C were found in fresh peripheral blood from adult blood donors. Moreover, CD94/NKG2A surface expression was transiently detected upon in vitro stimulation of CD94/NKG2C(+) NK cells in the presence of irradiated allogeneic PBMC or rIL-12. A similar effect was observed upon coculture of NKG2C(+) NK clones with human CMV-infected autologous dendritic cell cultures, and it was prevented by an anti-IL-12 mAb. NKG2A inhibited the cytolytic activity of NKG2C(+) NK clones upon engagement either by a specific mAb or upon interaction with a transfectant of the HLA class I-deficient 721.221 cell line expressing HLA-E. These data indicate that beyond its constitutive expression by an NK cell subset, NKG2A may be also transiently displayed by CD94/NKG2C(+) NK cells under the influence of IL-12, providing a potential negative regulatory feedback mechanism. The Journal of Immunology, 2009, 182: 829-836.
引用
收藏
页码:829 / 836
页数:8
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