A highly sensitive long-wavelength fluorescence probe for nitroreductase and hypoxia: selective detection and quantification

被引：117

作者：

Guo, Ting ^{[1
]}

Cui, Lei ^{[1
]}

Shen, Jiaoning ^{[1
]}

Zhu, Weiping ^{[1
]}

Xu, Yufang ^{[1
]}

Qian, Xuhong ^{[1
]}

机构：

[1] E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 92期

基金：

国家高技术研究发展计划(863计划);

关键词：

TUMOR HYPOXIA; FAR-RED; ACTIVATION; SUBSTRATE; STRATEGIES; REPORTER; ENZYME; OXYGEN; DYES;

D O I：

10.1039/c3cc45367g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel long-wavelength fluorescence probe NBP has been developed for the detection of nitroreductase (NTR) and hypoxia. NBP could be activated by NTR at 0.1 mu M to release the fluorophore NBF and significant changes in fluorescence emission at 658 nm were observed. This feature makes it advantageous for imaging hypoxic cells with minimal endogenous interference.

引用

收藏

页码：10820 / 10822

页数：3

相关论文

共 48 条

[1] Application of a microfluidic reactor for screening cancer prodrug activation using silica-immobilized nitrobenzene nitroreductase [J].

Berne, Cecile ;

Betancor, Lorena ;

Luckarift, Heather R. ;

Spain, Jim C. .

BIOMACROMOLECULES, 2006, 7 (09) :2631-2636

[2] Exploiting tumour hypoxia in cancer treatment [J].

Brown, JM ;

William, WR .

NATURE REVIEWS CANCER, 2004, 4 (06) :437-447

[3]

BRYANT C, 1991, J BIOL CHEM, V266, P4119

[4] TYPE-I NITROREDUCTASES OF ESCHERICHIA-COLI [J].

BRYANT, DW ;

MCCALLA, DR ;

LEEKSMA, M ;

LANEUVILLE, P .

CANADIAN JOURNAL OF MICROBIOLOGY, 1981, 27 (01) :81-86

[5] Exploiting the Reversible Covalent Bonding of Boronic Acids: Recognition, Sensing, and Assembly [J].

Bull, Steven D. ;

Davidson, Matthew G. ;

Van den Elsen, Jean M. H. ;

Fossey, John S. ;

Jenkins, A. Toby A. ;

Jiang, Yun-Bao ;

Kubo, Yuji ;

Marken, Frank ;

Sakurai, Kazuo ;

Zhao, Jianzhang ;

James, Tony D. .

ACCOUNTS OF CHEMICAL RESEARCH, 2013, 46 (02) :312-326

[6] A NOVEL CONNECTOR LINKAGE APPLICABLE IN PRODRUG DESIGN [J].

CARL, PL ;

CHAKRAVARTY, PK ;

KATZENELLENBOGEN, JA .

JOURNAL OF MEDICINAL CHEMISTRY, 1981, 24 (05) :479-480

[7] Fluorescent and luminescent probes for detection of reactive oxygen and nitrogen species [J].

Chen, Xiaoqiang ;

Tian, Xizhe ;

Shin, Injae ;

Yoon, Juyoung .

CHEMICAL SOCIETY REVIEWS, 2011, 40 (09) :4783-4804

[8] Design of Anticancer Prodrugs for Reductive Activation [J].

Chen, Yu ;

Hu, Longqin .

MEDICINAL RESEARCH REVIEWS, 2009, 29 (01) :29-64

[9] A New Prodrug-Derived Ratiometric Fluorescent Probe for Hypoxia: High Selectivity of Nitroreductase and Imaging in Tumor Cell [J].

Cui, Lei ;

Zhong, Ye ;

Zhu, Weiping ;

Xu, Yufang ;

Du, Qingshan ;

Wang, Xin ;

Qian, Xuhong ;

Xiao, Yi .

ORGANIC LETTERS, 2011, 13 (05) :928-931

[10] Copper bis(thiosemicarbazone) complexes as hypoxia imaging agents: structure-activity relationships [J].

Dearling, JLJ ;

Lewis, JS ;

Muller, GED ;

Welch, MJ ;

Blower, PJ .

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2002, 7 (03) :249-259

← 1 2 3 4 5 →