Subunit stoichiometry of a heteromultimeric G protein-coupled inward-rectifier K+ channel

被引：72

作者：

Silverman, SK

Lester, HA

Dougherty, DA

机构：

[1] CALTECH,DIV BIOL 15629,PASADENA,CA 91125

[2] CALTECH,DIV CHEM,PASADENA,CA 91125

[3] CALTECH,DIV CHEM ENGN,PASADENA,CA 91125

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1996年 / 271卷 / 48期

关键词：

D O I：

10.1074/jbc.271.48.30524

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the stoichiometry of the heteromultimeric G protein-coupled inward-recitfier K+ channel (GIRK) formed from GIRK1 and GIRK4 subunits. Multimeric GIRK constructs with several concatenated channel subunits were expressed in Xenopus oocytes. Coexpression of various trimeric constructs with different monomers clearly showed that the functional chan nel has stoichiometry (GIRK1)(2)(GIRK4)(2). Efforts to establish a preferred arrangement of subunits around the channel pore suggest that more than one arrangement may be viable.

引用

页码：30524 / 30528

页数：5

共 24 条

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