Culture conditions of Roseobacter strain 27-4 affect its attachment and biofilm formation as quantified by real-time PCR

被引：35

作者：

Bruhn, JB

Haagensen, JAJ

Bagge-Ravn, D

Gram, L

机构：

[1] Danish Inst Fisheries Res, Dept Seafood Res, DK-2800 Lyngby, Denmark

[2] Tech Univ Denmark, Bioctr, Ctr Biomed Microbiol, DK-2800 Lyngby, Denmark

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 04期

关键词：

D O I：

10.1128/AEM.72.4.3011-3015.2006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The fish probiotic bacterium Roseobacter strain 27-4 grows only as rosettes and produces its antibacterial compound under static growth conditions. It forms three-dimensional biofilms when precultured under static conditions. We quantified attachment of Roseobacter strain 27-4 using a direct real-time PCR method and demonstrated that the bacteria attached more efficiently to surfaces during static growth than under aerated conditions.

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收藏

页码：3011 / 3015

页数：5

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