Culture conditions of Roseobacter strain 27-4 affect its attachment and biofilm formation as quantified by real-time PCR

被引:35
作者
Bruhn, JB
Haagensen, JAJ
Bagge-Ravn, D
Gram, L
机构
[1] Danish Inst Fisheries Res, Dept Seafood Res, DK-2800 Lyngby, Denmark
[2] Tech Univ Denmark, Bioctr, Ctr Biomed Microbiol, DK-2800 Lyngby, Denmark
关键词
D O I
10.1128/AEM.72.4.3011-3015.2006
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The fish probiotic bacterium Roseobacter strain 27-4 grows only as rosettes and produces its antibacterial compound under static growth conditions. It forms three-dimensional biofilms when precultured under static conditions. We quantified attachment of Roseobacter strain 27-4 using a direct real-time PCR method and demonstrated that the bacteria attached more efficiently to surfaces during static growth than under aerated conditions.
引用
收藏
页码:3011 / 3015
页数:5
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