Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR

被引：45

作者：

Guilbaud, M

de Coppet, P

Bourion, F

Rachman, C

Prévost, H

Dousset, X

机构：

[1] ENITIAA, Lab Microbiol Alimentaire & Ind, F-44322 Nantes, France

[2] INRA, Unite Fonct Digest & Nutr Humaine, F-44026 Nantes, France

[3] Assoc ASEPT, Laval, PQ, Canada

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 04期

关键词：

D O I：

10.1128/AEM.71.4.2190-2194.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 X 10(2) CFU/cm(2).

引用

页码：2190 / 2194

页数：5

共 23 条

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