Molecular characterization of the PhoP-PhoQ two-component system in Escherichia coli K-12:: Identification of extracellular Mg2+-responsive promoters

被引：138

作者：

Kato, A ^{[1
]}

Tanabe, H ^{[1
]}

Utsumi, R ^{[1
]}

机构：

[1] Kinki Univ, Dept Agr Chem, Nara 6318505, Japan

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 17期

关键词：

D O I：

10.1128/JB.181.17.5516-5520.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We identified Mg2+-responsive promoters of the phoPQ, mgtA, and mgrB genes of Escherichia coli K-12 by S1 nuclease analysis. Expression of these genes was induced by magnesium limitation and depended on PhoP and PhoQ. The transcription start sites mere also determined, which allowed us to find a (T/G)GTTTA direct repeat in their corresponding promoter regions.

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页码：5516 / 5520

页数：5

相关论文

共 24 条

[1] AUTO-REGULATION OF THE ESCHERICHIA-COLI CRP GENE - CRP IS A TRANSCRIPTIONAL REPRESSOR FOR ITS OWN GENE [J].

AIBA, H .

CELL, 1983, 32 (01) :141-149

[2] COMPLETE SEQUENCE OF PSC101 [J].

BERNARDI, A ;

BERNARDI, F .

NUCLEIC ACIDS RESEARCH, 1984, 12 (24) :9415-9426

[3] TRANSPOSITION OF GAMMA-PLACMUS IS MEDIATED IN THE A-PROTEIN ALTERED AT ITS CARBOXY-TERMINAL END [J].

BREMER, E ;

SILHAVY, TJ ;

WEINSTOCK, GM .

GENE, 1988, 71 (01) :177-186

[4] TRANSPOSITION AND FUSION OF LAC GENES TO SELECTED PROMOTERS IN ESCHERICHIA-COLI USING BACTERIOPHAGE-LAMBDA AND BACTERIOPHAGE-MU [J].

CASADABAN, MJ .

JOURNAL OF MOLECULAR BIOLOGY, 1976, 104 (03) :541-555

[5] MOLECULAR GENETIC-ANALYSIS OF THE ESCHERICHIA-COLI PHOP LOCUS [J].

GROISMAN, EA ;

HEFFRON, F ;

SOLOMON, F .

JOURNAL OF BACTERIOLOGY, 1992, 174 (02) :486-491

[6]

GROISMAN EA, 1995, 2 COMPONENT SIGNAL T, P319, DOI DOI 10.1128/9781555818319

[7] Transcriptional regulation of Salmonella virulence: A phoQ periplasmic domain mutation results in increased net phosphotransfer to phoP [J].

Gunn, JS ;

Hohmann, EL ;

Miller, SI .

JOURNAL OF BACTERIOLOGY, 1996, 178 (21) :6369-6373

[8] PhoP-PhoQ activates transcription of pmrAB, encoding a two-component regulatory system involved in Salmonella typhimurium antimicrobial peptide resistance [J].

Gunn, JS ;

Miller, SI .

JOURNAL OF BACTERIOLOGY, 1996, 178 (23) :6857-6864

[9]

HOHMANN EL, 1994, PHOSPHATE IN MICROORGANISMS, P120

[10] MOLECULAR ANALYSIS OF THE ESCHERICHIA-COLI PHOP-PHOQ OPERON [J].

KASAHARA, M ;

NAKATA, A ;

SHINAGAWA, H .

JOURNAL OF BACTERIOLOGY, 1992, 174 (02) :492-498