Comparison of Stain-Free gels with traditional immunoblot loading control methodology

被引：115

作者：

Colella, Alex D. ^{[1
]}

Chegenii, Nusha ^{[2
]}

Tea, Melinda N. ^{[1
]}

Gibbins, Ian L. ^{[3
]}

Williams, Keryn A. ^{[1
]}

Chataway, Tim K. ^{[2
]}

机构：

[1] Flinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia

[2] Flinders Univ S Australia, Flinders Prote Facil, Dept Human Physiol, Bedford Pk, SA 5042, Australia

[3] Flinders Univ S Australia, Dept Anat & Histol, Bedford Pk, SA 5042, Australia

来源：

ANALYTICAL BIOCHEMISTRY | 2012年 / 430卷 / 02期

基金：

英国医学研究理事会;

关键词：

Immunoblot; Loading control; Stain-Free gel; Western blot; Semiquantitative;

D O I：

10.1016/j.ab.2012.08.015

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Loading controls are necessary for semiquantitative Western blotting to compensate for loading errors. Loading control methods include the reprobing of membranes with an antibody against a constitutively expressed protein or staining the membrane with a total protein stain. We compared the loading control performance of recently released Stain-Free (SF) gels with Sypro Ruby (SR) and reprobing using beta-actin. SF gels demonstrated superior performance in that they were faster, required fewer steps and consumables. and allowed the quality of electrophoresis and Western transfer to be assessed before committing to costly and time-consuming Western blots. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：108 / 110

页数：3

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